BRAF anticorps

N° du produit ABIN965693

L’anticorps Souris Monoclonal anti-BRAF a été validé pour WB, ELISA et IHC. Il convient pour détecter BRAF dans des échantillons de Humain. Il y a 3+ publications disponibles.

Aperçu rapide pour BRAF anticorps (ABIN965693)

Antigène: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BRAF est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 1H12F1-1H12G10-1F12F11C9

Détail du produit anti-BRAF anticorps

Purification: Ascitic fluid

Immunogène: Purified recombinant fragment of BRAF expressed in E. Coli.

Isotype: IgG1

Information d'application

Indications d'application: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Stockage

Format: Liquid

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-BRAF (ABIN965693)

Kim, Giuliano, Turner, Gaffney, Umetani, Kitago, Elashoff, Hoon: "Lymphatic mapping establishes the role of BRAF gene mutation in papillary thyroid carcinoma." dans: Annals of surgery, Vol. 244, Issue 5, pp. 799-804, (2006) (PubMed).

Terai, Matsuda: "The amino-terminal B-Raf-specific region mediates calcium-dependent homo- and hetero-dimerization of Raf." dans: The EMBO journal, Vol. 25, Issue 15, pp. 3556-64, (2006) (PubMed).

Noda, Kato, Yoshikawa, Arai, Togashi, Nagai, Konishi, Miki: "Frequent involvement of ras-signalling pathways in both polypoid-type and flat-type early-stage colorectal cancers." dans: Journal of experimental & clinical cancer research : CR, Vol. 25, Issue 2, pp. 235-42, (2006) (PubMed).

Détails sur BRAF

Antigène: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Autre désignation: BRAF

Sujet: BRAF(V-raf murine sarcoma viral oncogene homolog B1 ) is the main effectors recruited by GTP-bound Ras to activate the MEKMAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439).B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK),it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers.The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi,primary cutaneous melanomas and colorectal cancers.
Synonyms: BRAF

ID gène: 673

Pathways: Signalisation MAPK, Signalisation RTK, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy